~The History of 30 Crapboxes etc ~
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go and find out the signalling or whatever cascade that signals the iNOS gene expression then work from thereOriginally posted by starblue:er.. honestly, i dunno wad you're talking about...
and hor.. i dunno by wad mechanism LPS induces iNOS gene expression. somewhere in there NK-kuppa B is involved but really dunno a sht lah. so i dunno if it forms a complex with the dna or it signals via a secondary messenger or it induces a transcriptional factor or wadever it is among thousands of effects lah.... haiz.. liddat how right? -
o.O transcription factors a necessary for gene expression. if u wanna induce expression further, one way is to temper with the TFs.Originally posted by ndmmxiaomayi:*faints*
You want to induce gene expression but the transcriptional factor is there for what? -
and you are another one..man..all these genes..Originally posted by ndmmxiaomayi:*faints*
You want to induce gene expression but the transcriptional factor is there for what?
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ah.. so it was politics... rather associated with sociology doesnt it??? wait... wad do u major in again? wad year are u in now??Originally posted by LazerLordz:can I be your tutor?
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I dread..dread...indeed.Originally posted by StarPuppy:wah...i wonder how u get graded on that -
PS. Year 1.Originally posted by starblue:ah.. so it was politics... rather associated with sociology doesnt it??? wait... wad do u major in again? wad year are u in now??
Can't help it if my lecturer's books are sold in Kinokuniya and Amazon.com..
but he rants a heck of a lot..
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another one wad???Originally posted by LazerLordz:and you are another one..man..all these genes..
lil ant got study biology??? fascinating subject isnt it??? lil ant going poly/JC or in already? -
majoring in wad? PS??? o.OOriginally posted by LazerLordz:PS. Year 1.
Can't help it if my lecturer's books are sold in Kinokuniya and Amazon.com..
but he rants a heck of a lot.. -
Orh. Didn't know that.Originally posted by starblue:o.O transcription factors a necessary for gene expression. if u wanna induce expression further, one way is to temper with the TFs.
*flips books* -
/faintsOriginally posted by hisoka:go and find out the signalling or whatever cascade that signals the iNOS gene expression then work from there
do u have any idea of the extent of cascades in signalling pathways? o.O most of them will have nothing to do with LPS anyway and i will be wasting time searching from there.
haiz.. nvm, i'll think of something... at least the quantification, elucidation and real-time PCRing protocols can just take from the lab protocols. -
Fascinating. But hor, me poly year 1 student. School holidays end will be Year 2. Not studying science, but IT.Originally posted by starblue:another one wad???
lil ant got study biology??? fascinating subject isnt it??? lil ant going poly/JC or in already? -
erm... actually TFs are not covered in O or A levels.Originally posted by ndmmxiaomayi:Orh. Didn't know that.
*flips books*
so its ok if u dunno this. so u take biology is it? -
Yeah, in secondary school only.Originally posted by starblue:erm... actually TFs are not covered in O or A levels. so its ok if u dunno this. so u take biology is it? -
i think theres this procedure to complex dna and liposacchdarides together then use that complex to transfect mah. so just transfect twith the gene lorOriginally posted by starblue:/faints
do u have any idea of the extent of cascades in signalling pathways? o.O most of them will have nothing to do with LPS anyway and i will be wasting time searching from there.
haiz.. nvm, i'll think of something... at least the quantification, elucidation and real-time PCRing protocols can just take from the lab protocols. -
Yeah, minor in Cultural Studies, and an unofficial minor in film..(not recognized)Originally posted by starblue:majoring in wad? PS??? o.O -
you mean like...... take a iNOS gene and complex with LPS to do like a recombinant... thing. and then transfect it back to the mouse cell and hope that the cell's mechanisms will recognise the foreign gene and inducer?? o.OOriginally posted by hisoka:i think theres this procedure to complex dna and liposacchdarides together then use that complex to transfect mah. so just transfect twith the gene lor -
see la, more tinkering with animals, and you get all these animal problems.Originally posted by starblue:you mean like...... take a iNOS gene and complex with LPS to do like a recombinant... thing. and then transfect it back to the mouse cell and hope that the cell's mechanisms will recognise the foreign gene and inducer?? o.O
runs away -
win le.... only O level biology still come and question me..Originally posted by ndmmxiaomayi:Yeah, in secondary school only.
so, what do u study in IT??? did i mention computers dun really mix well with starblue? -
yupz. thats how they do those "cancer-striken" rats also. only worse cos after that must do some intebreeding or something if i am not wrongOriginally posted by starblue:you mean like...... take a iNOS gene and complex with LPS to do like a recombinant... thing. and then transfect it back to the mouse cell and hope that the cell's mechanisms will recognise the foreign gene and inducer?? o.O -
oh... cool... cultural studies is part of sociology or is it a department all by itself?Originally posted by LazerLordz:Yeah, minor in Cultural Studies, and an unofficial minor in film..(not recognized) -
erm..... those knockout mice is using constructs that are purely DNA leh.... like, purely gene mutation and stuff ya know.. not realy complexing with other things...Originally posted by hisoka:yupz. thats how they do those "cancer-striken" rats also. only worse cos after that must do some intebreeding or something if i am not wrong -
ah well... we can only blame the mice for having a very similar immune system to humans...Originally posted by LazerLordz:see la, more tinkering with animals, and you get all these animal problems.
[size=small]runs away/size] -
got complexing also i think, but maybe not those knock out bah.Originally posted by starblue:erm..... those knockout mice is using constructs that are purely DNA leh.... like, purely gene mutation and stuff ya know.. not realy complexing with other things...
i do know what i suggested they do it for bacteria haha -
Nope.It's under Office of Programmes.Originally posted by starblue:oh... cool... cultural studies is part of sociology or is it a department all by itself?
http://www.fas.nus.edu.sg/oop/undergrad5_3i.htm -
Oi, you think I never read books is it? You think I stuck at O Levels Biology nia?Originally posted by starblue:win le.... only O level biology still come and question me..
so, what do u study in IT??? did i mention computers dun really mix well with starblue?
Year 1 study all kinds of crap not related to IT. Year 2 starts programming and whatever things related to IT. Year 3 then go into specializations. I planned to take IT Security as my specialized subject.
I don't know computers don't mix well with you. Well, now that I know, I will be more careful with the terms.